phrynocephalus hongyuanensis meaning in Chinese

红原沙蜥

The full coding regions of bdnf genes were amplified from the genome of tylototriton taliangensis , phrynocephalus hongyuanensis , japalura splendida and cyclophiops major , respectively by pcr with the primers designed on the sequence of human bdnf gene . the pcr products were cloned into the vector pucis of esherichia coli . sequence analysis showed that the coding regions of three reptiles are the same ( 741 bp ) in length and these bdnf genes encode a peptide of 246 amino acid residues while that of tylototriton taliangensis is 744 bp in length and encodes a peptide of 247 amino acid residues
根据已有的人bdnf结构基因的全长序列设计了一对引物，利用pcr技术分别从大凉疣螈( tylototritontaliangensis ) 、红原沙蜥( phrynocephalushongyuanensis ) 、丽纹龙蜥( japalurasplendida )和翠青蛇( cyclophiopsmajor )的基因组dna中扩增到目的dna片段，并将其分别克隆到大肠杆菌载体中，然后对所获得的阳性克隆进行测序。